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KMID : 0545119980080050501
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 5 p.501 ~ p.508
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Isolation of Novel Alkalophilic Bacillus alcalophilus subsp. YB380 and the Characteristics of Its Yeast Cell Wall Hydrolase
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Yeo, Ik Hyun
Han, Suk Kyun/Yu, Ju Hyun/Bai, Dong Hoon
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Abstract
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An alkalophilic microorganism (strain YB380), which produces yeast cell wall hydrolase extracellulary, was isolated from Korean soil. The rod-shaped cells were 0.3∼0.4 by 2∼4μm long, motile, aerobic, gram-positive, and spore-forming. The color of the colony was light yellow. The temperature range for growth at pH 9.0 was 25 to 45℃, with optimum growth at 35℃. The pH range for growth at 35℃ was 8 to 11 with an optimum pH of 9.0. Therefore, the strain YB380 is an obligate alkalophile. The 16S rRNA of strain YB380 has a 99% sequence similarity with that of Bacillus alcalophilus. On the basis of physiological properties, cell wall fatty acid composition, and phylogenetic analysis, we propose that the isolated strain is Bacillus alcalophilus. The yeast cell wall hydrolase from Bacillus alcalophilus subsp. YB380 has been purified and partially characterized. The molecular weight was estimated to be 27,000 daltons with an optimum temperature and pH of 60℃ and 9.0, respectively. The N-terminal amino acid sequence of the enzyme was analyzed as Gln-Thr-Val-Pro-Trp-Gly-Ile-Asn-Arg-Val.
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